Sep 21, 2023
CHEM 4310 - Analytical Chemistry II: Chromatography and Spectroscopy
Survey of chromatographic and spectroscopic methods of analysis. Topics include liquid-liquid extractions, partition chromatography, ion-exchange, gas chromatography, high pressure liquid chromatography, exclusion chromatography, electrophoresis, atomic absorption, atomic emission, molecular absorption and molecular absorption and molecular emission and X-ray methods of analysis.
Requisites: C- or better in (CHEM 2410 and (3510 or 4530))
Credit Hours: 3
Repeat/Retake Information: May be retaken two times excluding withdrawals, but only last course taken counts.
Lecture/Lab Hours: 3.0 lecture
Grades: Eligible Grades: A-F,WP,WF,WN,FN,AU,I
- Calculate the energy of radiation from its wavelength, frequency, or wavenumber and interconvert between these quantities.
- Describe how spectra can be collected and analyzed.
- Know how to analyze the data derived from different separation techniques to perform qualitative and quantitative anlayses.
- Understand and use the Beer-Lambert law to find the amount of radiation absorbed at a given concentration or to find the molar absorption coefficient.
- Understand how to classify chromatographic methods and the general applications for each method.
- Understand the basic principals of chromatography, including equilibrium theory and kinetics.
- Understand the basic principles of atomic and molecular spectroscopy in terms of the quantization of energy levels and transitions between them.
- Understand the basic principles of mass spectrometry including the most common types of mass spectrometers, the origin of fragment ion spectra and some common interfaces (e.g. GC-MS, LC-MS).
- Understand the basic principles of spectroscopic measurements, the types of instruments used for each type of measurement and the performance characteristics of different spectroscopic devices.
- Understand the origin and measurement of NMR spectra.
- Understand the various instrumentation components required to conduct a given separation and detection, and be able to explain how each component operates.
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