CHEM 4310 - Analytical Chemistry II: Chromatography and Spectroscopy

• Calculate the energy of radiation from its wavelength, frequency, or wavenumber and interconvert between these quantities.
• Describe how spectra can be collected and analyzed.
• Know how to analyze the data derived from different separation techniques to perform qualitative and quantitative anlayses.
• Understand and use the Beer-Lambert law to find the amount of radiation absorbed at a given concentration or to find the molar absorption coefficient.
• Understand how to classify chromatographic methods and the general applications for each method.
• Understand the basic principals of chromatography, including equilibrium theory and kinetics.
• Understand the basic principles of atomic and molecular spectroscopy in terms of the quantization of energy levels and transitions between them.
• Understand the basic principles of mass spectrometry including the most common types of mass spectrometers, the origin of fragment ion spectra and some common interfaces (e.g. GC-MS, LC-MS).
• Understand the basic principles of spectroscopic measurements, the types of instruments used for each type of measurement and the performance characteristics of different spectroscopic devices.
• Understand the origin and measurement of NMR spectra.
• Understand the various instrumentation components required to conduct a given separation and detection, and be able to explain how each component operates.