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Oct 04, 2024
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CHEM 4880 - Microscopy and Imaging
This course is a survey of microscopy techniques used in research and forensic settings, with primary emphasis on optical-based microscopy but also a brief overview of electron microscopy and other non-optical techniques. Microscopy techniques covered in this course include: brightfield, dark field, polarized light, differential interference contrast, confocal, fluorescence, Raman, transmission electron microscopy, and near field. Additional topics discussed in this course include: imaging and spectroscopy through the microscope, standard optical components used in microscopy, analyzing microscopy data, and preparing microscopy data for a peer-reviewed publication or presentation.
Requisites: C or better in CHEM 3510 or 4540 or Permission
Credit Hours: 3
Repeat/Retake Information: May be retaken two times excluding withdrawals, but only last course taken counts.
Lecture/Lab Hours: 3.0 lecture
Grades: Eligible Grades: A-F,WP,WF,WN,FN,AU,I
Learning Outcomes: - Students will be able to describe the basic components utilized in optics, including: polarizers, filters, objectives, condensers, gratings, mirrors, prisms, light sources, and detectors
- Students will be able to explain the differences between common detectors used in microscopy
- Students will be able to illustrate how detector and microscope settings (e.g. exposure time, gain, light intensity, filters, polarizers, etc.) impact data collection
- Students will be able to describe the working principles for the standard formats of optical microscopy, namely: Brightfield, Darkfield, Polarized Light, Interference, Differential Interference Contrast, Raman, and Fluorescence Microscopies
- Students will be able to describe the working principles of advanced microscopy techniques, namely: Confocal, Atomic Force, Near Field, and Electron Microscopies
- Students will be able to explain the differences between optical and advanced microscopies
- Students will be able to determine the best type(s) of microscopy to use, based on the type of data sought and the type of sample being studied
- Students will be able to analyze imaging data using ImageJ and other software packages
- Students will be able to design effective figures for data collected via microscopy
- Students will be able to interpret published microscopy data
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